The solute carrier proteins (SLCs) comprise a superfamily of transporters controlling the import and export of molecules across membranes. With more than 450 members, SLCs are the largest family of transporters encoded by the human genome. Genetic studies have highlighted the roles that SLC transporters play in human health, and in common and rare diseases. Despite this, SLCs have long been under-explored and indeed less than 5% of all the SLC transporters are currently targeted by approved drugs.
The development of SLC functional assays for running high-throughput screening campaigns represents a fundamental approach for the discovery of novel inhibitors and activators of SLC transporters for therapeutic purposes.
Axxam has applied its consolidated experience and expertise in assay development to develop functional assays for many different SLCs, applying multiple and diverse technologies including, but not limited to, methods based on radiometric, optogenetic, and genetically encoded sensor detection.
In this webinar, case studies of assay development for several SLCs, belonging to many different families, will be shown and critical points illustrated.
Keywords:
SLCs, assay development, HTS, functional assays, optogenetics, genetically encoded sensors, radiometric, FLIPR, Pherastar, manual patch, RESOLUTE
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