High-Throughput Screening

Axxam has a long history and proven experience in High-Throughput Screening (HTS). We provide access to four fully automated state-of-the-art screening stations designed to run biochemical and cell-based assays in 384 well format, using a variety of detection technologies including luminescence, fluorescence, absorbance, HCS and radiometric readouts. The most appropriate screening strategy is selected for any screening campaign according to the specific target’s features and characteristics. This approach ensures the highest probability for a successful HTS with meaningful results.

Download Axxam HTS Brochure

High Content Screening (HCS) for phenotypic assays can be run on an OPERA®-based platform that includes fully automated “on-the-flight” imaging analysis.
Radiometric screening can be conducted on a CyBio centric screening station for scintillation proximity assays, whereas FlashPlate® assays are run on a semi-automated screening station.

Download Radiometric Screening flyer

We have also the possibility of accommodating Biosafety Level 2 organisms on one of the available screening stations.

We conduct hit finding programs using either compound collections available at Axxam or any customer’s library that is provided to us. We have excellent compound storage facilities and state-of-the-art compound handling and tracking.

For running an HTS, clients can access our assay development services and ready-to-go assays, or transfer their own validated assays to us.

HTS services include the following:

  • Assay adaptation to the specific screening conditions
  • Pilot screening performed on a sub-set of compounds
  • Primary screening
  • Data analysis for primary hit identification
  • Hit confirmation
  • Activity Determination (EC50 /IC50determination)
  • Preliminary SAR analysis

Our expertise and know-how in curated data analysis guarantees full exploitation of HTS results. Data are reported to clients in the form of GeneData Screener® visualizations.