Optogenetics applied to HTS
- Light-gated Channelrhodopsin-2 was used to modulate NCX1 sodium-calcium exchanger as well as CaV1.2, CaV1.3 or CaV3.3 calcium channels and to identify state-dependent blockers; very recently, voltage-activated sodium channels were also paced by ChR2, obtaining highly improved sensitivity towards NaV channel modulators and blockers
- HCN1 and HCN2 channels were activated either by light activated bPAC adenylyl cyclase or by light hyperpolarizing tools
- Genetically encoded indicators for voltage or calcium were used for single- or multi-color detection of KIR2.3, TMEM16A, NaV1.5, CaV1.2, CaV3.3, ASIC, TRPM2 and muscarinic receptor activity; moreover, new dynamic and sensitive cAMP sensors were used for Gs and Gi-coupled GPCR assays
FLIOP - Fluorescence Imaging and Optical Pacing
Optogenetics in Electrophysiology
How can we support your research programs?
We offer construction of optogenetic cell lines (both with light actuators and light sensors), which can be transferred to our
clients or run as part of contract research studies at Axxam, including HTS campaigns with clients’ and/or Axxam compound collections.