Webinar Summary:

Cell function depends on a multitude of dynamic protein-protein interactions (PPIs) organized in complex networks and intricately orchestrated in time and space. In turn, dysregulation of specific PPIs is known to cause or contribute to disease, rendering PPIs important targets for drug intervention. The perception on targeting intracellular PPIs with small molecules has witnessed remarkable changes over the last two decades. Considered “undruggable” for a long time, success stories derived, for example, from covalent binders and new molecular mode of actions, like targeted protein degradation, have resulted in a renaissance of PPIs in discovery project portfolios. Today, a large spectrum of different assay techniques is available to probe small molecule modulation of PPIs.

In our webinar, we will focus on the particular strength of cell-free, biochemical assays in dissecting molecular recognition events to enhance assay resolution identifying also weakly interfering compounds. Finally, we briefly discuss how this process can be supported by computational methodologies in the future.

Keywords:

Interaction assays; High-throughput screening; Hit validation; TR-FRET; Fluorescence polarization; Generative Machine Learning; Virtual Screening; Virtual High-Throughput Screening; Library Design.

Speakers:

• Jörg Hüser, CSO, Axxam

• Daniele Carettoni, Head of Biochemistry, Axxam

• Davide Sala, Computational chemist, Axxam